Transcriptomic Analysis and Functional Gene Expression in Different Stages of Gonadal Development of Macrobrachium rosenbergii

In order to decipher the functional genes and reveal molecular mechanism of gonadal development in Macrobrachium rosenbergii, a comparative transcriptome analysis was performed on testes ovaries at different developmental stages. A total 146,537 unigenes with an N50 2008 bp average length 1144 were obtained from sequencing raw data via quality control denovo assembly. Identification differentially expressed (DEGs) showed that there 339 468 DEGs among stages ovaries, respectively, 7993 between ovaries. The KEGG enrichment identified 13 candidate pathways related development, including insulin synthesis, oocyte maturation, steroid biosynthesis, which involved biological processes such as regulation hormone metabolism, sex cell proliferation amino acid metabolism. above insulin-like growth factor 1 receptor (IGF1R), heat shock protein 90 (Hsp 90), cyclooxygenase (COX) highly during yolk indicating these might be accumulation oogenesis. Meanwhile, calmodulin (CaM) other spermatogenesis, suggesting play important role spermatogenesis. Ten signaling pathway, CRQ, COX, APP, Cdc42, Hsd17b12, Art-1, Hsp70, Hsp90, PRMT1, GP, selected for real-time quantitative PCR (RT- qPCR) validate data, results RT- qPCR consistent RNA-Seq data. present findings provide new insights into M. rosenbergii.